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KMID : 0371319940470030307
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Journal of the Korean Surgical Society
1994 Volume.47 No. 3 p.307 ~ p.314
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The Role of Cell Surface Molecule(ICAM-1) in Murine Heart Lung Allograft
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Abstract
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The intercellular adhesion molecule (ICAM-1) in tissue as well as in circulation is known to act at an initial activation stage of immune cells as an inducer in immune-target cell interaction. Like class II antigen expression, the concern of
ICAM-1
in
diverse inflammatory reaction suggest that the study of ICNM-1 might reveal revela the early immune response.
In order to define the role of ICAM-1 in the early rejection following organ transplant, we analyzed cellular immune reaction relating to the ICAM-1 expression in graft and spleen in heart-lung transplanted mice.
Graft survival after heart-lung transplantation from BALB/C(H-2d) to CBA (H-2K) mice was 8.5±2.5days.
The splenocyte cytotoxicity was 10.5% in unsensitized host and peak response was noticed on post op day (6 30.60%) and then decreased lower than the control level on post op day 8 (4.94%).
Immunohistochemical study of ICAM-1 expression showed peak level on day 5 in splenic capsule, nonspecific finding in white pulp and maimal appearance of hematopoietic precursor cell on day 7 in red pulp of spleen.
In transplanted heart ICAM-1 positive cells were found in epicardium even in non transplanted heart, but they progressed gradually from the epicardium to myocardium and then endocardium. ICAM-1 expression in graft endothelical cell appeared on
post
operative day 2 and maximal response on post operative day 7.
Compared with the inflammatory cell infiltration starting from day 5, ICAM-1 positive cells in the graft appeared earlier.
Our experimental results showed compatible findings of lymphocyte cytotoxicity with the expression of ICAM-1 positive cells in spleen and graft prior to rejection following organ transplantation.
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KEYWORD
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